Abstract
Bio diesel fuel(BDF) was purified by column chromatography and the carbon 14(C-14) content was successfully measured by liquid scintillation counting(LSC). BDF was dissolved in hexane (10 wt%), and was purified by the column chromatography using an activated clay. Then the purified BDF was separated from hexane by evaporation under the boiling point of hexane. The purified BDF with toluene scintillator was measured by LSC. The biomass ratio obtained by LSC was in good agreement with that obtained by accelerator mass spectrometry.