2010 Volume 59 Issue 11 Pages 637-658
Nuclear Magnetic Resonance(NMR) is widely used method for metabolic analysis in biological systems. This would be attributed to the characteristic features of NMR;1) that's sample can be applied for various physical states, such as gases/solutions/solids, and including non-invasive measurements for cells/tissues/organs/individuals;2) although its information has selectivity in atomic level, observed spectra contains tremendous information regarding molecular structure, dynamics and quantities. In this review, we tried to discuss how technology developments of stable isotope labeling and its efficient analysis of multi-dimensional NMR spectra can contribute for promotion of metabolic analysis, like as protein NMR developed similar manner during the last century.
