RADIOISOTOPES
Online ISSN : 1884-4111
Print ISSN : 0033-8303
ISSN-L : 0033-8303
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Analysis of Glutamine in a Rice Seedling Plant Supplied with 15N, 13C Doubly Labeled Tracer
Naoto NIHEISayaka MASUDAKeitaro TANOIHiroki RAITomoko M. NAKANISHI
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JOURNAL OPEN ACCESS

2012 Volume 61 Issue 11 Pages 539-544

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Abstract
To study the uptake of glutamine in a rice seedling, a double-labeled (15N, 13C) glutamine was supplied for an hour and the glutamine in the seedling was analyzed using the high performance liquid chromatography equipped with the ion trap mass spectrometer. The mass spectrum peak of 15N- and 13C-glutamine was detected both in underground and above-ground part of the rice, suggesting that glutamine itself was absorbed from the root without decomposition at rhizosphere and was transferred to the above-ground part.
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© 2012 by Japan Radioisotope Association
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