Investigation of a Quick and Convenient Method Using Thin-layer Chromatography to Measure the Radiochemical Purity of ^99mTc-labeled Radiopharmaceuticals

Abstract

As a quality control of ^99mTc-labeled radiopharmaceuticals, a quick and convenient method of radiochemical purity(RCP) measurement were investigated. In this study, two methods to measure the RCP of ^99mTc-hydroxymethylene diphosphonate(^99mTc-HMDP) and ^99mTc-human serum albumin(^99mTc-HSA) were proposed.
The RCP of ^99mTc-HMDP was measured by thin-layer chromatography(TLC) using a mixture of 1M ammonium chloride, 1M carbamide, and acetic acid(70:28:2) as a solvent and a high-performance thin-layer chromatography cellulose plate. Development time taken by this method was a quarter of the time taken by the method using a mixture of 2M ammonium chloride, 10M carbamide, and acetic acid(49:49:2)as a solvent and a thin-layer cellulose chromatography plate without loss of measurement accuracy. The RCP of ^99mTc-HSA was measured by paper chromatography using 100% acetone as the solvent. Development time taken by this method was one sixteenth of the time taken by the method using 75% methanol as a solvent without loss of measurement accuracy.