RADIOISOTOPES
Online ISSN : 1884-4111
Print ISSN : 0033-8303
ISSN-L : 0033-8303
Article
Investigation of a Quick and Convenient Method Using Thin-layer Chromatography to Measure the Radiochemical Purity of 99mTc-labeled Radiopharmaceuticals
Noriyo YOKOTSUKAHiroshi TOMIZAWAHiroshi SASAKI
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JOURNALS OPEN ACCESS

2012 Volume 61 Issue 7 Pages 353-364

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Abstract

As a quality control of 99mTc-labeled radiopharmaceuticals, a quick and convenient method of radiochemical purity(RCP) measurement were investigated. In this study, two methods to measure the RCP of 99mTc-hydroxymethylene diphosphonate(99mTc-HMDP) and 99mTc-human serum albumin(99mTc-HSA) were proposed.
The RCP of 99mTc-HMDP was measured by thin-layer chromatography(TLC) using a mixture of 1M ammonium chloride, 1M carbamide, and acetic acid(70:28:2) as a solvent and a high-performance thin-layer chromatography cellulose plate. Development time taken by this method was a quarter of the time taken by the method using a mixture of 2M ammonium chloride, 10M carbamide, and acetic acid(49:49:2)as a solvent and a thin-layer cellulose chromatography plate without loss of measurement accuracy. The RCP of 99mTc-HSA was measured by paper chromatography using 100% acetone as the solvent. Development time taken by this method was one sixteenth of the time taken by the method using 75% methanol as a solvent without loss of measurement accuracy.

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© 2012 by Japan Radioisotope Association
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