Effect of Fasted Rat Liver Cell Fractions on Cholesterol Biosynthesis from Acetate-1-¹⁴C and Mevalonic Acid-2-¹⁴C

Abstract

Cholesterol biosynthesis from acetate-1-¹⁴C and mevalonic acid-2-¹⁴C in normal or 48 hours fasted rat liver cell fractions was observed.
1. Biosynthesis in normal or 48 hours fasted microsome fractions was not changed.
2. Biosynthesis from acetate in fasted soluble fraction was inhibited, but biosynthesis from mevalonic acid was not changed.
3. Biosynthesis from acetate and mevalonic acid in fasted mitochondria fractions was depressed, and biosynthesis from acetate in normal mitochondria fraction was inhibited, but from mevalonic acid was not changed.
4. Biosynthesis from acetate and mevalonic acid in 48 hour fasted liver slice was inhibited, but from acetate in 24 hour fasted slice, was inhibited, but from mevalonic acid, was not inhibited.
5. These observations are suggested that the inhibitory substance resides in mitochondrial fraction and fasted soluble fraction.