Abstract
The method of preparing tritium labelled compounds has been studied using high voltage discharge and microwave discharge. This method can be widely applied to various compounds including complicated ones which are not easily prepared with usual techniques.
Labelled compounds with high specific activities have been found to be easily prepared in a short period in comparison to the contact method or recoil labelling method. The microwave discharging technique is found to be much superior to the high voltage discharging technique because of a simple discharge reactor tube and smaller production of impurities which have to be removed.
Tritium labelled acetic acid was prepared with various conditions. For the purification of reactants, precise distillation and gas chromatographic techniques were applied after esterification of acetic acid. Labile tritium was completely removed while these purifications of the reactant were made.
