Abstract
UIBC was determined rapidly by using a new simple device consists of an outer test tube, which is also used for counting, and an inner tube having a rubber piston ring, which has a sponge or cotton filter inside the ring.
The serum and ferric ammonium citrate 59FeFe solution are added and incubated for 15 minutes in the outer tube.
Amberlite IRP-67 ion exchange resin powder is added to the iron serum solution mixture, and kept for one minute. Thereafter the inner tube is inserted and pushed down. Then the serum solution appears above the filter and the resin remains under the piston ring. The serum solution is removed and the resin is washed with water three times in a minute by pushing and pulling the inner tube. The washed resin in the tube is counted in a well-type scintillation counter. In reverse, if the resin is added onto the piston ring and the inner tube is removed, the serum solution for counting is obtained.
The use of this device enables the rapid determination of UIBC without the procedures as pipetting, centrifugation, and mixing.
