RADIOISOTOPES
Online ISSN : 1884-4111
Print ISSN : 0033-8303
ISSN-L : 0033-8303
Adsorption Characteristics of 14C-Labeled Alanine, Aspartic Acid and Adenosine Triphosphate by Metal-chelating Resins
Toshio ISHIYAMATadao MATSUNAMISetsuko SHIBATAYoshihide HONDA
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1987 Volume 36 Issue 3 Pages 122-125

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Abstract

(1) Adsorption properties of 14C-alanine, 14C-ATP (adenosine triphosphate) and 14C-aspartic acid on the metal-chelating resins were determined and found that the Cu (II) -Chelex 100 and Fe (III) -Unicel-lex UR10, Fe (III) -Chelex 100 chelating resins were highly effective for the adsorption of 14C-alanine and 14C-ATP, respectively. (2) Desorption rate of 14C-ATP from the Fe (III) -Unieellex UR10 and Fe (III) -Chelex 100 resins was somewhat higher than the case of 14C-alanine, probably because the coordination bonds of Cu-alanine might be stronger than those of Fe-ATP. Thus, 14C-labeled organic compounds such as 14C-alanine and 14C-ATP of a low activity concentration (3.7 mBq/ml) (1 × 10-7μCi/ml) in aqueous solution may be measured with liquid scintillation counter after pre-concentration by use of the Fe (III) -and Cu (II) -chelating resin columns.

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