Adsorption Characteristics of ¹⁴C-Labeled Alanine, Aspartic Acid and Adenosine Triphosphate by Metal-chelating Resins

Abstract

(1) Adsorption properties of ¹⁴C-alanine, ¹⁴C-ATP (adenosine triphosphate) and ¹⁴C-aspartic acid on the metal-chelating resins were determined and found that the Cu (II) -Chelex 100 and Fe (III) -Unicel-lex UR10, Fe (III) -Chelex 100 chelating resins were highly effective for the adsorption of ¹⁴C-alanine and ¹⁴C-ATP, respectively. (2) Desorption rate of ¹⁴C-ATP from the Fe (III) -Unieellex UR10 and Fe (III) -Chelex 100 resins was somewhat higher than the case of ¹⁴C-alanine, probably because the coordination bonds of Cu-alanine might be stronger than those of Fe-ATP. Thus, ¹⁴C-labeled organic compounds such as ¹⁴C-alanine and ¹⁴C-ATP of a low activity concentration (3.7 mBq/ml) (1 × 10^-7μCi/ml) in aqueous solution may be measured with liquid scintillation counter after pre-concentration by use of the Fe (III) -and Cu (II) -chelating resin columns.