Abstract
The effects of leukapheresis and plateletpheresis on normal donors using Celltrifuge and Haemonetics Model 30 to obtain large quantities of granulocytes and platelets were investigated. The procedure to obtain granulocytes using Haemonetics were performed over a 2-hr period using 6% hydroxyethyl starch (M. W. 45×104, 20×104) and the donor received an intravenous injection of Solu-Cortef 100 or 200 mg before each procedure.
Changes in many laboratory data (less than 20% decrease) were observed. However, it was considered that they occurred due to hemodilution by saline infusion and plasma expansion by 6% HES. Except these minor changes, white blood cells and granulocytes increased significantly in donors with Solu-Cortef injection. About 20 to 30% decrease of platelet count was observed in donors with plateletpheresis. It was noteworthy that 29% decrease of serum inorganic phosphorus was appeared in 10 cases treated without 6% HES, while in 15 cases treated with 6% HES 50% decrease (P<0.01) was shown.
The leukapheresis and plateletpheresis using Celltrifuge, or Haemonetics have been shown without severe side-effects. Numbness in lips (11.2%) and extremities (6.2%) was observed in the Haemonetics procedure.
