抄録
Taking the fluorescence spectra is a useful method for the study of green leaf, because the fluorescence spectra give us the information about microstructure of photosystems of the leaf. The spectra of fluorescence induced by UV Ar-laser lights had a peak around 450 nm (F 450), a shoulder around 530 nm (F 530) and peaks of chlorophyll at 687 nm (F 687) and 741 nm (F 741). In order to examine the transverse distribution of the blue-green (450 nm, 530 nm) and the chlorophyll fluorescence (687 nm, 741 nm) within the leaf tissue induced by UV and visible laser lights projected on the intact leaves of camellia tree, a micro-fluorescence imaging (MFI) system was devised using a microscope, a CCD camera with an image intensifier, a microcomputer and laser light sources (Ar laser and He-Ne laser).
The fluorescence of 450 nm and 530 nm were observed at the epidermal cell, and the chlorophyll fluorescence (687 nm and 741 nm) at the palisade cell. Because the epidermal cell does not contain chlorophyll, fluorescence (450 nm and 530 nm) do not relate to the photosynthetic reaction directly. It is considered that these results are due to compounds in the leaves accumulated according to physiological characteristics of each plant species. We took the fluorescence spectra of young and old leaves of camellia tree by LIF system which was composed of Ar-lasers (351-364 nm, 488 nm and 514.5 nm) and a He-Ne laser (632.8 nm) for excitation, and an optical multichannel spectrometer. From the result of these, the relations between LIF spectra of plant leaves and transverse distribution of blue-green and chlorophyll fluorescence within leaf tissue were described. We conclude that imaging of transverse distribution of fluorescence in the intact plant leaves is a powerful method for the study of LIF spectra to examine activity and ecological status of plants by remote-sensing.
