Actinomycetologica
Online ISSN : 1881-6371
Print ISSN : 0914-5818
ISSN-L : 0914-5818
Original Articles
Development of a PCR/Southern dot blot based detection system for the presence of genes involved in beta-lactam biosynthesis
M. KrallisR. Kirby
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JOURNAL FREE ACCESS

1998 Volume 12 Issue 1 Pages 29-36

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Abstract

Screening of actinomycetes and fungi for genes involved in beta-lactam production can be carried out using a technique involving both PCR with conserved primers to the isopenicillin N synthase gene and a Southern dot blotting. This technique was successful with nine out of nine known beta-lactam producing Actinomycetes tested and three out of six known beta-lactam producing Fungi tested. It proved unable to detect the isopenicillin N synthase gene in the two Flexibacter beta lactam producing eubacteria strains tested. It also identified the presence of potential beta-lactam production genes in two known none beta-lactam producing actinomycetes and one out of fourteen novel newly isolated actinomycetes. This technique has potential for use in commercial screens for novel beta-lactam producers.

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© 1998 The Society for Actinomycetes Japan
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