Cloning, Sequencing, and Characterization of the Xylanase-Encoding Gene, svxA, from Streptomyces viridosporus T7A and its Expression in Escherichia coli

抄録

A xylanase-encoding gene from lignocellulolytic Streptomyces viridosporus T7A was isolated, expressed, cloned and sequenced. The gene was isolated by screening an Escherichia coli cosmid genomic library of S. viridosporus T7A genomic using a substrate overlay method. One xylanase expressing cosmid clone contained a 30 kb DNA insert. The xylanase gene was subsequently subcloned on a 2 kb PstI fragment and sequenced. Analysis of the nucleotide sequence revealed a 915 bp open reading frame coding for a 329 amino acid protein with a calculated molecular weight of 33,550 Da. This gene, designated svxA (Genbank accession number AF198618), shows high sequence homology to genes belonging to xylanase family G.