Abstract
This report deals with the effects of brown sugar and organic substrates on the mycelium productivity of a basidiomycetes strain of Agaricus blazei Murill mushroom under the submerged culture using 1L volume fermentor. The spawn was separated from the fruit body of the mushroom grown on bagasse compost. Basic salt medium composed of NH4NO3, KH2PO4, MgSO4 and FeSO4 was used. Additional substrates were sucrose, wheat bran, brown sugar, barley grain and bagasse. Cultures were cultivated under 30°C and 72h duration with stirring and forced aeration. Yields of mycelium tended to increase in the order of the additions of wheat bran, brown sugar, barley grain and bagasse. However, the organic medium composed of yeast extract, polypepton, bagasse and sucrose showed the highest mycelium of 6.69g/L as biomass. As for the effect of organic medium added with sucrose, the β-glucan content in the cultured medium was 4.14mg per g of the harvested mycelium while that in the liquid used to wash the harvested mycelia was about 0.03mg/g, and that in the dried mycelium was 5.25mg/g of its own mass, respectively. Therefore, the content rate of β-glucans in the whole culture was about 9.42mg per g of the harvested mycelium. From the difference (1.75mg/g) between the β-glucan content in the harvested mycelium (5.25mg/g) and that in the mycelium extracted with hot water (3.50mg/g), it was showed that the extraction rate of the glucans from the harvested mycelium was about 33.3%.
