抄録
Mass spectrometer is one of the most promising instruments for proteomics and metabolomics. It is not only applied for the protein and small molecule identification, but also for the complicated post-translational modification analysis, biomarker discovery from the quantitative analysis between the multiple samples, and so forth. Hitachi High-Technologies produced a novel liquid chromatograph mass spectrometer (NanoFrontier LD), which may identify and quantitate the proteins and peptides of interest. The improvements in the detection system brought the dynamic range and resolution over 5,000 and 10,000, respectively, enabling the high-resolution determination. Alternative collision-induced dissociation technique enhances the information abundance, and functional addition to the software advances the analysis efficiency. Furthermore, we also developed the pretreatment methodology, for enzymatic digestion and affinity purification, by applying the unique protein immobilization technique presently used in protein microarrays. These technical approaches and details were described and discussed.
