質量分析装置による次世代プロテオミクス解析のための新しいメソッド

抄録

Based on MALDI-TOF mass spectrometry technique, Bruker Daltonics is developing the CLINPROT^TM system solution for the clinical proteomics including biomarker search and Imaging MS. For the biomarker search from body fluid like blood serum or plasma, simple sample purification using CLINPROT^TM magnetic beads kit is effective. After MS measurements, ClinProTools^TM software is useful to search the biomarker from many complicated spectra. On the other hands, direct monitoring of protein distribution in a tissue using Imaging MS technique is attractive. Bruker Daltonics MALDI Molecular Imager^TM is a total solution of Imaging MS using MALDI-TOF-MS. It includes flexImaging^TM software, which has wizard-like interface simplifying the operations and settings from automatic measurements to data processing/evaluation. MALDI Molecular Imager^TM is also a part of CLINPROT^TM system.
Proteomics analysis based on mass spectrometry also is expected for post translation modification analysis. Electron transfer dissociation (ETD) in a non-linear Paul trap has been introduced as a new fragmentation technique, which avoids internal parent ion heating. Induced by the electron transfer the intermediate peptide radical cation fragments randomly at each amino acid position of the peptide backbone, which is particularly suitable for PTM identification and analysis of large multiply charged peptides. HCTultra PTM discovery system^TM has CID- and ETD-MS/MS both, which takes easy CID-MS/MS data as proteomics analysis and ETD-MS/MS data as PTM analysis. Top-down proteomics by FT-ICR-MS is very powerful technique for identification of protein and PTM analysis. Bruker Daltonics is developing Top-down analysis system solution.