抄録
Electrophoretic analyses on blood plasma of children are so rare in our country that we studied the plasma protein analysis on 22 healthy children, 14 cases in 6 years (group A) and 8 yases in 2-4 years old (group B), with Tiselius' apparatus using Schlieren-diagonal method (Type HT-B Hitachi made).
Pooled plasma were dialyzed for 16-20 hours, before electrophoresis, against 1/20M phosphate buffer.
Experimental conditions were as follows; electric voltage 90-140V., electric current 9-10mA., time of electrophoresis 40-80 minutes, temperature at 16°C., Diagonal-Slit angle 25°.
The results are shown in the next Table.
In comparison with those values of the adult which were reported by many authors,
1) total protein showed no remarkable difference,
2) alubumin some decrease, and
3) globulin no remarkable difference.
3) globulin no remarkable difference.
4) α-Globulin was not able to be compared with that of the adult, because there wag significant difference between group A and B. This may well be due to difference of time of electrophoresis.
5) β-Globulin showed no remarkable differnce,
6) γ-globulin some increase, and
7) fibrinogen no remarkable difference.
