DISC電気泳動を用いたイタイイタイ病患者の尿蛋白について

抄録

A characteristic kidney disorder is commonly seen in Itai-itai disease in addition to the severe bone damage. Hence, urinary proteins of Itai-itai disease patients were analyzed by disc electrophoresis in order to diagnose this disease early and to distinguish it from other kidney diseases.
The results are summarized as follows.
1) Five different bands, designated as B-1 to 5 according to the decreasing order of the anodic mobility, were commonly detected in urinary proteins of Itai-itai disease. This pattern was specific for the disease and was provisionally named as I (Itai-itai disease) type.
2) Three different bands, one corresponding in mobility to serum albumin and two other slower migrating bands, were seen in urinary proteins in other kidney diseases, while the urinary protein from healthy adults was composed solely of albumin. These patterns were provisionally named as K (kidney disease) and N (normal) type respectively.
3) Among the five bands seen in the I-type pattern, B-3 and 4 specifically appeared in Itai-itai disease and seemed to be important in distinguishing I-type from K or N-type.
4) Six protein fractions, prealbumin, albumin, α₁, α₂, β, and γ-globulins were prepared by cellulose acetate electrophoresis from a Itai-itai disease patient's urine and were utilized in disc electrophoresis to identify the above described five bands. It was revealed that B-1 was composed of α₁-globulin, B-2 of albumin, B-3 of α₁ and α₂-globulins, and B-4 and B-5 of α₂ and β-globulins, respectively.
These results indicate that the disc electrophoretic analysis of urinary proteins is a useful screening test in the early diagnosis of Itai-itai disease. It also provides a clear distinction of this disease from other kidney diseases.