1981 Volume 24 Issue 4 Pages 335-340
Separation of immunoglobulin-linked LDH in patient serum was performed by high performance liquid chromatography and post-column enzyme reactor.
In the case of high speed anion exchange chromatography with IEX-525-QAE (4.0 i. d.×50mm), abnormal chromatograms of IgA-linked LDH and IgG-linked LDH were obtained. Minor difference in separation mechanisms were observed between electrophoretic and anion exchange chromatographic methods.
In the case of high speed gel permeation chromatography with TSK-5, 000 PW (7.6 i. d.×600mm) and double TSK-3, 000 SW (7.6 i. d.×600mm), IgA-linked LDH was completely separated from normal size of LDH and determination of each LDH activity was performed. Moreover, IgG-linked LDH was separated to two high molecular weight of LDH. This finding will suggest that IgG-linked LDH will be different in its binding forms from IgA-linked LDH.
