Site of Biosynthesis of Tyrosinase in Mammalian Melanocyte

Abstract

Tyrosinase was purified from B16 mouse melanoma cells. Anti-tyrosinase antiserum was prepared using rabbits. Polysomes were prepared from the mouse melanoma cells and were found to be active in in vitro protein synthesis. The tyrosinase-synthesizing Polysomes were isolated from both free and membrane-bound Polysomes by the immunoprecipitation method. Results of our study indicate that both free and membrane-bound polysomes are responsible for synthesizing tyrosinase.