Analysis of urinary proteins from patients of renal disease by miro two-dimensional electrophoresis

Abstract

The urinary proteins from 19 patients of renal disease were analyzed with micro two-dimensional electrophoresis in the absence of denaturing agents. The two-dimensional electrophoretic patterns of the urinary proteins resembled with those of plasma proteins, although the contents of high molecular weight plasma proteins were low. The patterns of the patients with nephrotic syndrome were characterized by the high content of α₁-acid glycoprotein. Comparing the urinary protein patterns, differences in the contents of G_C-globulin, prealbumin, α₂ HS-glycoprotein, IgG polymer and unidentified low molecular weight proteins were demonstrated.