Abnormal apoproteinemia and its clinical significance

Abstract

The purpose of this study is to demonstrate clinical significances, lipid composition of plasma lipoproteins, and plasma apoprotein concentrations in the patients with abnormal apoproteinemia (two apo E-3 deficiency, one patients with apo E-5, and two hypoalphalipoproteinemias). Apo E isoform was analyzed by the analytical isoelectric focusing of apo-VLDL using 7.5% of polyacrylamide gel in each subject. Plasma apo A-I, A-II, B, C-II, and E concentrations were measured by the single radial immunodifusion methods. Lipid concentrations of each plasma lipoproteins were measured after the isolation by the ultracentrifuge. Two patients with apo E-3 deficiency were type IV and II hyperlipoproteinemia, and the latter was diabetic treated with sulfonylurea. The diabetic female had a cholesterol-rich VLDL, and her plasma apo E was 12.4mg/dl, whereas another one male showed normal ratio of VLDL-TC/TG and apo E concentration. One chronic thyroidism had apo E-5, and his plasma apo E was 5.2mg/dl. However, his plasma apo B concentration was significantly higher than that of the healthy volunteers. He had no evidence of cardiovascular disease. His daughter has hyperthyroidism, but her apo E isoform was normal and plasma apo E level was also normal. One familial hypercholesterolemia and one hypertriglyceridemia showed hypo α-lipoproteinemia. Their plasma apo A-I, II were significantly lower, and apo B were markedly higher than those of the control, respectively. It is possible that the lower concentration of HDL-cholesterol can be based on the decreased HDL₃-cholesterol rather than HDL₂-cholesterol. The paper discuss their back grounds of abnormal apoproteinemia.