SEIBUTSU BUTSURI KAGAKU
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
Volume 29, Issue 1
Displaying 1-13 of 13 articles from this issue
  • Mitsuo Hara
    1985 Volume 29 Issue 1 Pages 1-6
    Published: March 15, 1985
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
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  • Mitchel M. Yokoyama
    1985 Volume 29 Issue 1 Pages 7-13
    Published: March 15, 1985
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
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  • Seiichiro Yamasaki
    1985 Volume 29 Issue 1 Pages 15-16
    Published: March 15, 1985
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
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  • Ikunosuke Sakurabayashi
    1985 Volume 29 Issue 1 Pages 17-21
    Published: March 15, 1985
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
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  • Linda K. Curtiss
    1985 Volume 29 Issue 1 Pages 23-33
    Published: March 15, 1985
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
    Mouse monoclonal antibodies that bind either human apolipoprotein (apo) AI or human apo AII were characterized with respect to their binding of high density lipoprotein (HDL) particles in solution. The single apo AII-specific antibody bound 85% of 125I-HDL and 100% of soluble 125I-apo AII. However, none of the three apo AI-specific antibodies bound greater than 60% of either HDL or soluble apo AI. Limiting amounts of antibody or antigen, radioiodination of the ligands, and unavailability of the epitopes for reaction with antibody were not responsible for the incomplete binding of HDL and apo AI, suggesting the existance of immunochemical heterogeneity of apo AI as organized on HDL as well as free apo AI in solution. The validity of this heterogeneity was supported by demonstrating that: i) increased binding of HDL occurred when each of the apo AI antibodies were combined to from an oligoclonal antibody mixture, and ii) 100% binding of HDL occurred when two apo AI antibodies were combined with the single apo AII antibody. These studies demonstrated that all apo AII peptides in plasma HDL possess at least one epitope in common, whereas the apo A I peptides in HDL are immunochemically heterogenous.
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  • Isoelecric focusing for detection and characterization of apolipoprotein mutants
    Taku Yamamura
    1985 Volume 29 Issue 1 Pages 35-41
    Published: March 15, 1985
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
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  • Shunichi Koga, Yasuji Miyata
    1985 Volume 29 Issue 1 Pages 43-49
    Published: March 15, 1985
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
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  • Masato Ageta, Takaharu Taniguchi
    1985 Volume 29 Issue 1 Pages 51-56
    Published: March 15, 1985
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
    The purpose of this study is to demonstrate clinical significances, lipid composition of plasma lipoproteins, and plasma apoprotein concentrations in the patients with abnormal apoproteinemia (two apo E-3 deficiency, one patients with apo E-5, and two hypoalphalipoproteinemias). Apo E isoform was analyzed by the analytical isoelectric focusing of apo-VLDL using 7.5% of polyacrylamide gel in each subject. Plasma apo A-I, A-II, B, C-II, and E concentrations were measured by the single radial immunodifusion methods. Lipid concentrations of each plasma lipoproteins were measured after the isolation by the ultracentrifuge. Two patients with apo E-3 deficiency were type IV and II hyperlipoproteinemia, and the latter was diabetic treated with sulfonylurea. The diabetic female had a cholesterol-rich VLDL, and her plasma apo E was 12.4mg/dl, whereas another one male showed normal ratio of VLDL-TC/TG and apo E concentration. One chronic thyroidism had apo E-5, and his plasma apo E was 5.2mg/dl. However, his plasma apo B concentration was significantly higher than that of the healthy volunteers. He had no evidence of cardiovascular disease. His daughter has hyperthyroidism, but her apo E isoform was normal and plasma apo E level was also normal. One familial hypercholesterolemia and one hypertriglyceridemia showed hypo α-lipoproteinemia. Their plasma apo A-I, II were significantly lower, and apo B were markedly higher than those of the control, respectively. It is possible that the lower concentration of HDL-cholesterol can be based on the decreased HDL3-cholesterol rather than HDL2-cholesterol. The paper discuss their back grounds of abnormal apoproteinemia.
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  • Yoshihiro Ohtaki, Hiro-o Watanabe, Michiteru Yoshida, Kensuke Shimura
    1985 Volume 29 Issue 1 Pages 57-65
    Published: March 15, 1985
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
    A method is described for the large scale isolation of nonhistone proteins from salt-urea dissociated pig thymus chromatin. After precipitating DNA in a dissociated chromatin solution with LaCl3 at pH 7.9, the chromosomal proteins in the supernatant were precipitated by raising the pH of the supernatant to 8.6. The chromosomal proteins in the precipitate were dissolved and separated into nonhistone proteins and histones by chromatography on SP-Sephadex C-25 using a sodium chloride stepwise elution. Almost all of the total nonhistone proteins in chromatin was eluted from the column at 0.25M and 0.3M NaCl.
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  • Kinue Ohi
    1985 Volume 29 Issue 1 Pages 67-73
    Published: March 15, 1985
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
    We studied clinical significance of alkaline phosphatase isozyme, ALP1, which appeared in sera of patients with malignant diseases. Incidence of ALP1 was high in lung, esophageal, bile duct, gallbladder cancer, malignant lymphoma and leukemia. In contrast it was low in thyroid, mammary, prostate cancer and myeloma. ALP1 often appeared in post operative stages and the activity of ALP1 increased within a month after operation of solid cancers and of abdominal benign diseases. In the cases of solid cancers ALP1 peak appeared within 6-16 days after operation. However, in the cases of abdominal benign diseases, its appeared 3 to 10 days after operation.
    We observed relationship between incidence of ALP1 and liver function or LDH isozyme in haematopoietic malignant diseases. In both normal and abnormal liver groups, the incidence of ALP1 was higher in the cases with tumor pattern LDH isozyme (increase in LDH2 and/or LDH3) than those with the normal pattern.
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  • Motoo Matsuda, Hideo Fujitani
    1985 Volume 29 Issue 1 Pages 75-81
    Published: March 15, 1985
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
    The U1-snRNP particles from the nuclear extracts of human KB cells and mouse teratocarcinoma embryoid bodies (OTT6050), purified by SLE-anti-(U1)RNP antibody affinity chromatography, were analyzed for their protein components. The purified human U1-snRNP was recovered from the column by elution with 2.5M MgCl2 at pH7.2 after the column had been washed with Tris-buffer containing DOC, Triton X-100 and 1.0M NaCl. The 3H amino acid-labeled protein components of human (KB) U1-snRNP resolved into three major bands with apparent molecular weights of 69, 000, 58, 000 and 35, 000 upon SDS-polyacrylamide gel electrophoresis.
    Highly purified U1-snRNP particles containing U1a and U1b RNAs as their sole nucleic acid moieties were obtained from the mouse teratocarcinoma embryoid bodies by the same procedure as used for purification of KB cell U1-snRNP. By gel electrophoresis, five major polypeptides, with apparent molecular weights of 80, 000, 76, 000, 72, 000 58, 000 and 39, 000, were detected associated with U1-snRNP(s) of embryoid bodies.
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  • Hisae Niitsu, Nori Nakayashiki, Reiko Kumagai, Syusaku Katsura
    1985 Volume 29 Issue 1 Pages 83-88
    Published: March 15, 1985
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
    Sweats were obtained from 54 healthy adult men in a sauna. Supernatants of the sweats after centrifugation were dialyzed with deionized water and lyopholized. The sweat samples dissolved in normal saline were tested by PAG-slab electrophoresis. The sweat samples obtained from the 52 men were stained with Coomassie brilliant blue R250 and those from the 54 men were with PAS. The results were as follows: All the sweat samples had one band corresponding with the electrophoretic mobility of serum albumin. Two bands seemed to be important components of sweats were in the α-globulin region of serum. Individual differences of electrophoretic patterns of the sweat samples were found in the regions of α-, β- and γ-globulins. Three glycoprotein components having a individually different mobility were observed in the pre-albumin region.
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  • Yukiharu Sumi, Yutaka Naoi, Takao Tanaka, Hitoshi Katayama
    1985 Volume 29 Issue 1 Pages 89-98
    Published: March 15, 1985
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
    Time sequential changes of various serum protein fractions of rabbits irradiated in a single dose of 800R of 60Co γ-ray, were evaluated by electrophoresis, immunoelectrophoresis and single radial immunodiffusion. The results are as follows;
    (1) The increase of serum albumin level was observed immediately after irradiation. However, serum albumin level decreased significantly afterwards. On the statistical analysis, significant correlation between serum albumin level and other protein fractions was recognized.
    (2) The significant decrease of α1-globulin level was encountered immediately following irradiation.
    (3) Time sequential changes of serum α2-globulin were opposite to those of serum albumin level.
    (4) The significant increase of β-globulin was recognized for certain period following irradiation.
    (5) The day of irradiation significant decrease of serum γ-globulin level was obtained in immediate and first day after irradiation.
    This phenomenon was thought to be related to IgG value.
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