Peptide mapping 法による Euhadra 属卵白腺蛋白質の解析
1986 年 30 巻 1 号 p. 75-82
詳細
1986 年 30 巻 1 号 p. 75-82
The protein constituents of albumen gland from Euhadra subnimbosa, E. peliomphala, and E. p. simodae were analyzed by SDS polyacrylamide gel electrophoresis. Furthermore, peptide maps of three species were studied by two-dimensional electrophoresis with high sensitive silver stain and standardized. A unique peptide map was obtaind from albumen gland of each species. The peptide maps of E. peliomphala and E.p. simodae were especialy similar.
Effect of the steroid hormone, 17β-estradiol, on protein synthesis of albumen gland (E. subnimbosa in hibernation) was analyzed by O'Farrell's 2-dimensional electrophoresis. Five peptide groups in peptide map, (No.5, 8, 9, 11, and 13), were commonly found in some individuals of 17β-estradiol injected snails. These five peptide groups were detected to a normal individual in feeding.
We suggested that the five peptide groups, (No.5, 8, 9, 11, and 13), inducibled by 17β-estradiol.
