生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
SDSポリアクリルアミドゲル電気泳動法によるラット血清蛋白質の定量性の検討
山根 明千葉 元〓
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ジャーナル フリー

1987 年 31 巻 3 号 p. 147-153

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Quantitative analysis of protein components in the rat serum was done by a SDS-PAG electrophoretic method. The protein components were quantified by measuring the areas under peaks of the densitometric diagram obtained by scanning the electrophoretic lane in the gel. No systematic changes in the peak areas were found among lanes in the same gel and scanning positions in the same lane. Relations between the amounts of samples and the peak areas were linear in all protein components. Variations between the two measurements made twice at a 10 day interval were relatively great. It is concluded that relative amounts of proteins could be measured fairly accurately in the same gel, that the amount of a protein component could be estimated by comparing the unknown sample with a standard one, and that it would be necessary to make corrections using a standard sample to compare proteins among different gels.
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© 日本電気泳動学会
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