Blotting 技術によるENAの解析
1988 年 32 巻 1 号 p. 1-6
詳細
1988 年 32 巻 1 号 p. 1-6
By using immunoblotting technique, antigenic structures of various U small nuclear RNPs (UsnRNPs) were analysed which reacted with autoantibodies in sera from patients with collagen diseases. Based on these antigenic structures of UsnRNPs, anti-U1RNP antibodies could be more clearly differentiated from anti-U1U2U4-6RNP [Sm] antibodies. However the reactivity of anti-U1RNP antibodies with each antigenic popypeptides of U1RNP showed marked heterogeneity. An anti- (U1U2) RNP antibody system was newly described with a possible clinical association of the system.
Immunoblotting technique was shown to be useful as a sensitive test for screening various anti-ENA antibodies in clinical laboratory. However the sensitivity of the test depended on the concentration of antigens bound to the nitrocellulose membrane.
