ヒポキシア関連基質添加による in vitro の LDH-hybridization における変化

抄録

It is well known that hybrids of LDH isoenzyme are formed in vitro from the mixture of LD-1 and LD-5. We have established the condition of medium to produce the hybrids, and examined the influences on the hybridization by the addition of substances concerning hypoxia. In the loading anerobic condition and in the addition of lactate and pyruvate, there were no significant effects on the hybridization and total enzyme activities. On the contrary, the addition of NADH had considerable effects upon the hybridization and enzyme activity. Namely, increasing LD-5 was markedly observed in the hybrid patterns, and the higher enzyme activity was significantly recognized for comparison with control. While, in the addition of NAD a slight increase of LD-1 was only observed in the hybrid pattern.