Turned gel electrophoresis of mouse liver ribosomes in polyacrylamide agarose composite gel

Abstract

Mouse liver ribosomes were analyzed by 1.8% polyacrylamide 0.5% agarose composite gel electrophoresis (7.5% cross linkage gel). When the ribosomal suspension mixed with agarose was applied to the gel slot, ribosomes were resolved in the composite gel. When turned gel electrophoresis in which gel concentration was the same for the second run, was carride out, ribosome bands from 40S subunits to the maximum hexamers were observed. This high resolution electrophoresis may be one of the most powerful tools available to analyze the non-denatured ribonucleoprotein particles and membrane components and to study the conformational change of ribosomes.