Computer simulation of electrophoretic transport
II. Modified general procedure and an application to isoelectric focusing of proteins using immobilized pH gradient
1994 年 38 巻 6 号 p. 417-423
詳細
1994 年 38 巻 6 号 p. 417-423
My computer simulation procedure was applied to isoelectric focusing of proteins using immobilized pH gradient formed by Immobilines. The results for small amount of proteins (micromole order of proteins on a medium 4cm long, 10cm wide and 0.1cm thick) agreed well with those of widely reported experiments, while simulations for very large amount (10 to 100 times the above amount) of proteins with slightly differing isoelectric points resulted in a pattern that could be expressed as zero-velocity isotachophoretic separation of proteins.
