金属キレートアフィニティを用いたリン酸化タンパク質のプロテオミクス

抄録

The comprehensive analysis of post-translational modification of protein is thought to be the most important object of biomedical proteomics in the post-genome era. Especially, protein phosphorylation is quite important process in the physiological regulation of metabolism, and the irregular phosphorylation might be resulted in various diseases including malignant tumors. However, the concentration of those regulatory phosphoprotein level is generally too low to be detected directly by the mass spectrometry in proteomics. Therefore, preliminary concentration of phosphopeptides by the method of immobilized iron affinity chromatography (IMAC) is necessary to achieve the sensitive detection. In this paper, the present status and the prospect of the method of IMAC are discussed.