Characterization of low molecular weight starch granule associated protein in common wheat by proteomic approaches

抄録

Starch granule proteins (SGPs) were extracted from wheat seeds and characterized using proteomic approaches: two dimensional electrophoresis (2-DE), 40cm long gradient sodium dodecyl sulfate (SDS) gel electrophoresis and electrospray ionization mass spectrometry. The majority of the low molecular weight (LMW) SGPs, especially in the range of 14-30kDa, was located on the surface of starch granule, and only a small number of LMW SGPs of molecular weight range from 30 to 55kDa was found within starch granules. The surface SGPs were mostly expressed in acidic, whereas the integral SGPs were expressed basic regions of 2-DE. Gene location of one surface SGP of approximately 14kDa was assigned on short arm of chromosome 4B. One of the LMW SGPs was identified by LC-ESI MS/MS endochitinase (30kDa). Intensity of this proteins was found to decrease during seed germination and seed maturation.