2004 Volume 48 Issue 3 Pages 99-103
One-dimensional sodium dodecyl sulfate polyacrylamido gel electrophoresis and mass spectrometry were used to make a catalogue of soluble proteins in the human vitreous humor (VH). From patients with diabetic retinopathy, totally 121 different proteins including seven angiogenesis modulated factors were identified. Semi-quantifications of pigment epithelium-derived factor, one of anti-angiogenic factors, were suggested not to decrease in VH of patients with diabetic retinopathy by western-blot.
It is well known to be a unique antibody against cancer specific proteins in cancer patient's serum. In lung cancer as well as the other cancers, SM. Hanash first identified anti-annexins autoantibodies that induce a humoral immune response (Pro. Natl. Acad. Sci. USA, 99: 9824-9829, 2001). We applied two-dimensional electrophoresis/western-blot/soft-ionization mass spectrometry to find cancer-specific proteins in patient's sera, which interact with the expressed proteins of adenocarcinoma cell. We found some cancer specific proteins by proteomics-based technique. Especially in adenocarcinoma patients and esophageal cancer patients we frequently found a specific protein, Aldehyde dehydrogenase, alpha-enolase and Phosphoglycinamide formyltransferase, which may be specific marker.