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Volume 48 (2004)
- Issue 4 Pages 123-
- Issue 3 Pages 81-
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Volume 48, Issue 3
Displaying 1-7 of 7 articles from this issue
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Tadashi Kawai2004 Volume 48 Issue 3 Pages 81-84
Published: September 15, 2004
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSProtein science has been advanced with new development of various separation and analytical techniques, and one of the most useful techniques at the beginning was various electrophoretic fractionation. In 1973, Watson & Crick reported on a structure of deoxyribonucleic acid, and then molecular biology has progressed rapidly. In 1991, the international cooperative project on human genome started, and ended in 2003, reporting more than 99% of the whole base sequence of human genome. However, the project has left most of human genes functionally unknown. Currently the major research in life science is aiming to determine biological functions of individual proteins as well as regulatory networks among many proteins, namely “proteomics”.View full abstractDownload PDF (737K) -
Mari Tabuchi, Yasuhiro Kuramitsu, Kazuyuki Nakamura, Yoshinobu Baba2004 Volume 48 Issue 3 Pages 85-87
Published: September 15, 2004
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSHigh-throughput with high quality proteome analysis is necessary for proteomics research and medical diagnosis. In this paper, we introduce the view of advanced microchip electrophoresis for clinical application in proteomics research. Protein size separation based on sodium dodecyl sulfate-gel electrophoresis (SDS-GE) requires denaturing, but we propose that denaturing is unnecessary for analysis by microchip electrophoresis. We attained dramatically improved sensitivity without compromising size determination by omitting the protein denaturing process. Protein expression of Jurkat cells during stress-shock induced apoptosis was readily analyzed using this system. We found that a food extraction effectively induced apoptosis of Jurkat cells. This system will accelerate the proteomics research and will be utilize for the medical diagnosis in future.View full abstractDownload PDF (497K) -
Tadashi Kondo2004 Volume 48 Issue 3 Pages 89-91
Published: September 15, 2004
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSTwo-dimensional polyacrylamide gel electrophoresis (2D-PAGE) has a great utility to develop clinical markers, as it uncovers many types of multiple proteins in quantitative, reproducible and high throughput manner. Current technological progress around 2D-PAGE, such as fluorescence dyes, bioinformatics, laser microdissection, enable more sophisticated and accurate proteomic study. For instance, the tumor marker for unknown-origin tumor will be developed by identifying the expression pattern of spots. Although numerous challenges still lie ahead, the future of proteomics, especially medicine-oriented proteomics, by 2D-PAGE looks promising.View full abstractDownload PDF (479K) -
Yasuhiro Kuramitsu, Kazuyuki Nakamura2004 Volume 48 Issue 3 Pages 93-97
Published: September 15, 2004
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSChronic infection with hepatitis C virus (HCV) is one of the most clearly established risk factors for hepatocellular carcinoma (HCC). The number of HCV-carriers in Japan is estimated to be 2 million and 32, 000 of HCC patients pass away every year. Therefore, it is important to clarify the mechanism involved in hepatocarcinogenesis by chronic HCV infection.
We performed proteomic studies using samples of tumor tissues and non-tumor tissues from HCC patients with HCV infection but not hepatitis B virus infection. This study using two-dimensional gel electrophoresis with MALDI-TOF-MS showed that HSP70 family such as GRP78, HSC70, GRP75 and HSP70.1, glutamime synthetase isoforms, alpha-enolase, phosphoglycerate mutase, and triosephosphate isomerase were increased but albumin, ferritin light chain, smoothelin, tropomyosin beta chain, arginase 1, aldolase B and ketohexokinase were decreased in the tumor tissues.View full abstractDownload PDF (4049K) -
Identification of angiogenesis modulated factors and antibody to cancer associated proteinsToyofumi Nakanishi2004 Volume 48 Issue 3 Pages 99-103
Published: September 15, 2004
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSOne-dimensional sodium dodecyl sulfate polyacrylamido gel electrophoresis and mass spectrometry were used to make a catalogue of soluble proteins in the human vitreous humor (VH). From patients with diabetic retinopathy, totally 121 different proteins including seven angiogenesis modulated factors were identified. Semi-quantifications of pigment epithelium-derived factor, one of anti-angiogenic factors, were suggested not to decrease in VH of patients with diabetic retinopathy by western-blot.
It is well known to be a unique antibody against cancer specific proteins in cancer patient's serum. In lung cancer as well as the other cancers, SM. Hanash first identified anti-annexins autoantibodies that induce a humoral immune response (Pro. Natl. Acad. Sci. USA, 99: 9824-9829, 2001). We applied two-dimensional electrophoresis/western-blot/soft-ionization mass spectrometry to find cancer-specific proteins in patient's sera, which interact with the expressed proteins of adenocarcinoma cell. We found some cancer specific proteins by proteomics-based technique. Especially in adenocarcinoma patients and esophageal cancer patients we frequently found a specific protein, Aldehyde dehydrogenase, alpha-enolase and Phosphoglycinamide formyltransferase, which may be specific marker.View full abstractDownload PDF (3748K) -
Kazuko Sakaguchi, Jun Suzuki, Masaki Tanaka, Fumiaki Akahori2004 Volume 48 Issue 3 Pages 105-109
Published: September 15, 2004
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSChanges in rat plasma proteins after administration of paraquat (PQ) were observed by micro two-dimensional polyacrylamide gel electrophoresis (Type II), under denaturing conditions. In the area of molecular weight lower than that of albumin, there were increases in four spots of plasma proteins, i.e., those at isoelectric points (pI) 5.0 and 5.9 with a molecular weight (MW) of ca. 54, 000, and 5.7 and 7.5 with a MW of ca. 15, 000, in the rats administered PQ. On the other hand, there were decreases in two spots of plasma proteins, i.e., those at pI 5.0-6.0 with a MW of ca. 54, 000 and 5.0 with a MW of ca. 18, 000. The protein spot at the pI 5.0 with a MW of ca. 18, 000 was identified as α2u-globulin (AUG) by MALDI-TOF/MS/MS.
Some rats administered PQ showed the marked decrease in AUG, and pathological findings of pulmonary fibrosis were also observed. These results suggest the involvement of AUG with PQ-induced pulmonary fibrosis.View full abstractDownload PDF (7086K) -
Hideyuki Kajiwara, Yoshio Sugimura2004 Volume 48 Issue 3 Pages 111-115
Published: September 15, 2004
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA new technique for tube-gel isoelectric focusing (IEF) using plastic tubes instead of glass tubes in the first dimension of two-dimensional gel electrophoresis (2-DE) was developed for proteomic analysis of both denatured and non-denatured proteins. The plastic tubes have advantages in handling gels over glass tubes. The plastic tubes were also applied successfully to native gel electrophoresis, blue-native gel electrophoresis, agarose gel IEF, acid-urea gel electrophoresis and cetyltrimethylammonium bromide (CTAB) gel electrophoresis for the first dimension of 2-DE.View full abstractDownload PDF (9077K)
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