抄録
Visualization and measurement of biomolecules based on an atomic force microscope (AFM) allow us to observe individual molecules in their native form. Because the measurement can be performed in buffer solution and it does not require fluorescence labeling or staining. This technique could be applied for the detection of bound site of MutS protein to DNA successfully. Recent developments in AFM technology such as a low noise nano-positioning sensor for closed-loop operation have opened the door to new era of single molecule manipulation. We demonstrate that we can take aim at a portion of DNA adsorbed on mica substrate and we can fish it up with an AFM tip.
