透析操作を省いた迅速電気泳動法について

抄録

A method for dispensing with the dialysis of serum and plasma samples prior to electrophoresis according to Tiselius' method in free solution has been described.
One volume of the sample was mixed with threee volumes of the phosphate buffer solution, and the mixture was analyzed by using a certain buffer solution (pH 7.9 and μ 0.15) as the supernatant in the electrophoretic run.
The electrolyte compositions of these buffer solutions were shown.
In cases where no pathological changes in the electrolyte composition of the blood cun be observed, a close agreement between electrophoretic analyses carried out accoring to this method and according to the conventional one with dialysis has been demonstrated on eight samples of the sera of healthy men and on eight samples of the sera and on four samples of the plasmas (obtained by means of heparinazed blood) of patients of various diseases.