Development of a robotic system interfaced to a microfl uidic device to isolate a single chromosome

抄録

Polyamine chromosomes in solution are used for various applications where the quality of the isolated chromosomes used as starting material is a key factor. Here we describe our current efforts for obtaining a single chromosome using microfl uidics and collecting using a robotic device. Beads of similar size to the hydrodynamic radius of chromosomes were used to characterize and describe the instrumentation development as well as to aid in future improvements as needed. The procedures described here have the potential to provide a rapid and efficient way to isolate highly pure human chromosomes as starting material for many applications thus offering exciting new possibilities.