Molecular Characterization of Entamoeba histolytica Isolates in Osaka City

抄録

The protozoan parasite, Entamoeba histolytica, is a causative agent of human amebiasis. Recent molecular studies show the genetic diversity among the isolates, and therefore identification of the isolates at the genotype level is important for control of the E. histolytica infection and for understanding the population structure of E. histolytica genotypes. In the present study, we identified the genotypes of the twenty-two isolates from patients in Osaka City by sequencing analysis of three polymorphic loci. Four genotypes (B, E, D, G) were found in locus 1-2, eight (A5, A5v, A6, A7, Cv, A6/Cv, A10/A5, A10/A6) in locus 5-6 and four (A, B, C, D, F) in chitinase. The following genotype at each locus was dominant type: genotype E in locus 1-2 (68%), A10/A5 in locus 5-6 (33%), B in chitinase (58%). Moreover, the isolates in Osaka City examined were classified into nine types (I to IX) based on the combination of genotypes. The present study showed the genetic polymorphism among the isolates in Osaka City and the possible presence of isolates peculiar to Osaka City.