Novel 3D in vitro model for evaluating vascular permeability

Abstract

Increased vascular permeability is a hallmark of inflammation accompanying tumor progression and diabetic retinopathy. For applying to anti-inflammatory drug testing and screening, we have newly developed an in vitro 3D microvasculature model to evaluate vascular permeability. In this model, we could measure and calculate extravasation pattern of FITCdextran fluorescence and determine endothelial barrier changes in response to thrombin, a known pro-inflammatory protease.