Abstract
In this paper, we focus on integrated approaches to liver tissue equivalent that can be finally used as substitute for those damaged original liver organ. To achieve the large implantable liver, a tissue construct of 500 cm3 in volume with in vivo comparable per-volume-based functionalities is necessary. This could hardly be realized by current technologies due to insufficient mass transfer problems in in vitro engineered tissues. We established a novel engineering methodology by integration of both bottom-up and top-down tissue engineering technologies, which provided an efficient way of arranging engineered liver tissue with improved mass transfer simultaneously from micro- and macro-scales, meanwhile addressing to a clinically relevant size with well recovered per-volume-based functions.
