Abstract
Cytochrome c3 has four hemes per molecule, and donates electrons to hydrogenase catalysing hydrogen evolution. In this study, Zn porphyrin intercalated cytochrome c3 was prepared in order to utilize high affinity of cytochrome c3 to hydrogenase in photoinduced hydrogen evolution systems. Photodriven reduction of Zn porphyrin intercalated cytochrome c3 occurs by visible light irradiation. An electron transfers from photoexcited Zn porphyrin to intrinsic Fe porphyrin. Procedures of Zn porphyrin was prepared by the intercalation of Zn porphyrin to apo type cytochrome c3. The apo type cytochrome c3 was obtained by removing one of four hemes in cytochrome c3. Photoinduced hydrogen evolution was observed by the irradiation, and photoinduced electron transfer occurs from Zn porphyrin to hemes and then to hydrogenase.
