Production of Paclitaxel in a Plant Cell Culture by In Situ Extraction with Sequential Refreshment of Water-Immiscible 1-Butyl-1-methylpyrrolidinium Bis(trifluoromethanesulfonyl)imide

抄録

We report here the effect of sequential refreshment of a water-immiscible ionic liquid, 1-butyl-1-methylpyrrolidinium bis(trifluoromethanesulfonyl)imide (P14TFSI), enhancing the production amount of paclitaxel with in situ extraction from an aqueous medium in a plant cell culture. The production amount of paclitaxel with refreshment of 2.5 vol% P14TFSI twice every 2 days was 8 times greater than that without the refreshment and 210 times greater than that of control culture in the absence of P14TFSI. The enhanced production amount of paclitaxel in the culture with P14TFSI might be due to the deceased feedback inhibition of paclitaxel and stress response against elevation of intracellular production of reactive oxygen species (ROS) derived from P14TFSI's abiotic stress.