Abstract
For taking an effective countermeasure based on measurements of toxicities of wastes, it is necessary to employ bioassays alongside leaching test. We examined leachability of organic compounds in 6 waste deposit samples, such as fly and bottom ashes, by standard and modified leaching tests. Leachability of organic compounds was much varied by the condition of leaching test. To evaluate the toxicity of waste samples, three different bioassays, in vitro cytotoxicity test using human cell lines, Daphnia acute immobilization test and Algae growth inhibition test, were applied to leaching test samples. The results of these bioassays were affected by osmolarity of the samples varied from 10 to 622 mOsm/kg. To evaluate the effect of osmolarity, osmolarityresponse curves of the three bioassays were collected. By using the standard curves, it was elucidated that significant toxicity was observed in leaching solutions of waste samples. Further bioassay can reveal the toxicity, which can be explained by the presence of organic compounds.
