Air-borne microbial diseases are reported in large numbers in hospitals. They are fungal allergic diseases of asthma and atopic dermatitis, pathogenic infections, and hospital-acquired infections. Practically, however, reports on actual air environmental condition in hospitals are few and the hospital designers do not understand the actuality. From a viewpoint of indoor microbial contamination and to construct the database providing indices for design and management of hospital buildings, the dynamic investigation was made on air-borne, settling, and adhering microbes inside general hospitals. This paper reports the result of comparison of the investigation results with the temperature/humidity conditions, existing indices and on the field investigation between the rooms in hospital equiped with the antibacterial performances in daily-changes, and differed from the last studies.