二, 三の集積培養菌による栄養塩類無添加での浸出実験

抄録

In this report, we describedo bservationso f behavioro n the long time leachinge xperimentsu sings ome enrichmentc ultures.
Our study was made by a conbinationo f the followingtw oexperiments;(1) a experimentw ithout containingn utrient except for energy source, which is the same as a method applied usually in a basic study of the pure culture, (2) a long time leachinge xperimentu nder pH 2 with a enrichmentc ulture.
The enrichment cultures were used for FeSO₄oxidation and S⁰leaching in the absence of nutritive salts. The relations between treatment time and product amount of Fe³⁺or SO₄^2-were linear.
The followingo bservationws ere obtaineda bout the behavioro f the enrichmentc ulturesi n these experiments.
1) It was supposed that the drop of apparent oxidation velocity of Fe²⁺at the high values of the ratio [Fe³⁺]/[Fe²⁺] resultedf rom the regulatorym echanismo f bacteria.
2) From the result of leachinge xperimentso f elementals ulfur, it was recognizedth at a enrichmentc ulture kept a constant activity for a long time.