2014 Volume 57 Issue 4 Pages 369-376
Objective: Ozone which generates OH radicals may assist the resurgence of the medical use of OH radicals. Ozone water and ozone gel may inhibit the effectiveness of OH radicals as an antibacterial agent. The purpose of this study was to investigate the bactericidal effects of ozone gel at different concentrations against key oral pathogens and the cytotoxicity to adult human gingival and periodontal ligament fibroblasts (HGF and HPDL).
Material and Method: Streptococcus mutans (ATCC25175), Streptococcus sanguinis (ATCC49296), Aggregatibacter actinomycetemcomitans (ATCC29522), and Porphyromonas gingivalis (W83, ATCC33277) were selected for the bactericidal tests. Each genus of bacteria was incubated with ozone gel for one minute at room temperature and their survival rates were measured by colony counting. HGF was obtained from the gingival tissue around extracted adult human teeth. Cytotoxicity of ozone gel against HGF and HPDL was studied by cell proliferation assessment with Alamar Blue assay.
Results and Conclusion: The results demonstrated that ozone gel concentration exceeding 125 ppm had significant bactericidal effects on A. actinomycetemcomitans and P. gingivalis (W83, ATCC33277). Ozone gel concentration of 500 ppm had significant bactericidal effects on S. sanguinis, but not on S. mutans. Compared with glycerol, ozone gel concentration of over 50 ppm showed significant inhibition of HGF and HPDL proliferation. These results suggest that ozone gel has prominent bactericidal effects against periodontal pathogens.
