1992 Volume 55 Issue 3 Pages 294-295
The staining ability of the monoclonal antibodies (MoAbs) E 8 and P 54 was evaluated by light and electron microscopy using formalin-fixed, paraffin-embedded sections and fresh frozen sections of oral squamous cell carcinomas. E 8 and P 54 produced positive staining in the cell membrane of normal and neoplastic stratified squamous epithelia. Although E 8 and P 54 microscopically reacted throughout the cell membrane, they showed different localizations ultrastructurally. E 8 reactivity was evident throughout the cell membrane of normal and neoplastic stratified squamous epithelia, but staining with P 54 was localized around the desmosomes. These MoAbs may be useful in the investigation of oral epithelium and squamous cell carcinoma.
