Substrate profile of Prevotella intermedia MA1 and MA1-V 2 β-lactamase

Abstract

The sensitivity of β-lactamase producer Prevotella intermedia (Pi) against β-lactams varies with the type of β-lactam. We studied the substrate profile of β-lactamase extracted from this organism. MIC of penicillins such as PCG, ABPC and MCIPC, and first generation cephems, such as CEX and CEZ, against Pi MA1-V2 was in the range of 25 to 200μg/ml. However, these values for second and the third generation cephems were in the range of 0.2 to 100μg/mg. Pi MA1-V2 β-lactamase hydrolyzed penicillins and first generation cephems except for MCIPC. However, second and the third generation cephems were not hydrolyzed by this β-lactam. These results suggest that Pi MA1-V2 shows high resistance against penicillins and first generation cephems, since it hydrolyzes these drugs with β-lactamase.