Detection of telomerase RNA, actin mRNA and granzyme mRNA in inflamed human gingiva

Abstract

We attempted to develop a detection system for human telomerase RNA (hTR), β-actin mRNA and granzyme A mRNA using a single section of human gingival tissue, and to discuss the relationship between immunohistochemical staining and RNA expression. One 4μm frozen section and one 4μm paraffin section of human gingiva were examined by RT-PCR and immunohistochemical staining. RT-PCR analysis of the three RNAs was accomplished by appropriate combination of the design and selection of primers. Actin and telomerase were strongly detected in all sections examined irrespective of the degree of inflammation in the gingival tissue, although expression of granzyme depended on the extent of the inflammation in the epithelial region. Samples with stronger inflammation had greater granzyme activity.