Immunohistochemical study of γδT-cells in periodontal disease

Abstract

We examined the production of monoclonal antibodies (MoAb) against human γδT cells to understand the role these cells play in the pathogenesis of periodontal disease. Stimulation of human peripheral lymphocytes with solid-phase anti-CD 3 antibody and soluble IL-2 produced a 100-500-fold increase that included about 50% γδT cells in the two week culture. These γδT cells were used as an antigen to produce the MoAb. Ten MoAbs were isolated and one of them, MoAb 1, was characterized immunohistochemically using gingival sections with periodontal disease, and compared with commercially available anti-γδT cell antibody.