7 Coordinated effect of IL-17 A and IL-27 on osteoclast differentiation of RANKL-stimulated RAW264.7 cells

Abstract

The macrophage cell line RAW264.7 is differentiated into osteoclasts by stimulation of receptor activator of nuclear factor κB ligand (RANKL). We examined the coordinated effect of IL-17 AandlL-27 on osteoclast differentiation of RANKL-stimulated RAW264.7 cells. The coordinated effect of IL-17 A and IL-27 decreased the number of multinuclear TRAP-positive cells, which are induced by RANKL. Phosphorylation of JNK, which was markedly induced by RANKL stimulation, was inhibited by the coordinated effect of IL-17 A and IL-27. These results suggest that the reduction of JNK phosphorylation, which is induced by the coordinated effect of IL-17 A and IL-27, may be a factor that suppresses the differentiation of osteoclast precursors into osteoclasts.