1 Effects of high glucose for hard tissue formation on type II diabetes model rat bone marrow cells in vitro

Abstract

Diabetes mellitus (DM) is one of the main etiology/risk factors of periodontal disease, and is intimately involved with the treatment of the disease. Although it has been reported that high blood glucose mediates the proliferation, differentiation and production of inflammatory cytokines from mesenchymal cells, there have been few reports on the effect of DM on periodontal regenerative therapy. We examined the biological effect of high blood glucose on the bone marrow mesenchymal stem cells (BMMSC) of Goto-Kakizaki (GK) rats. Cell culture experiments were performed on the BMMSC of the animals. The biological effects of the high glucose condition on the BMMSC was determined at four concentrations of culture solution by examining cell proliferation, cell differentiation and the production of inflammatory cytokines. We found that highly-concentrated glucose promotes cell proliferation and inhibits hard tissue differentiation and calcification in the BMMSC of GK rats. Differentiation depended on production of inflammatory cytokines like IL-6. The data suggests that a high glucose condition promotes cell proliferation while inhibiting hard tissue differentiation and calcification because of the inflammation by periodontal regeneration.